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AMED 再生医療実用化研究事業による臨床研究 重症下肢虚血に対する脱分化脂肪細胞(DFAT)を用いた細胞治療の実用化

AMED 再生医療実用化研究事業による臨床研究 重症下肢虚血に対する脱分化脂肪細胞(DFAT)を用いた細胞治療の実用化

DFAT細胞写真

業績

原著論文

  1. Yagi K, Kondo D, Okazaki Y, Kano K. A novel preadipocyte cell line established from mouse adult mature adipocytes. Biochemical and Biophysical Research Communications 321(4):967-974, 2004.
  2. Matsumoto T, Kano K, Kondo D, Iribe Y, Tanaka N, Matsubara Y, Sakuma T, Fukuda N, Satomi A, Otaki M, Ryu J, Mugishima H. Mature adipocyte-derived dedifferentiated fat cells exhibit multilineage potential. Journal of Cellular Physiology 215(1):210-222, 2008
  3. Ohta Y, Takenaga M, Tokura Y, Hamaguchi A, Matsumoto T, Kano K, Mugishima H, Okano H, Igarashi R. Mature adipocyte-derived cells, DFAT (de-differentiated fat cells) promoted functional recovery from spinal cord injury-induced motor dysfunction in rats. Cell Transplantation 17(8):877-886, 2008
  4. Nur R, Fukuda N, Matsumoto T, Jimabay M, Kano K, Yamamoto C, Maruyama T, Endo M, Matumoto K. Implantation of dedifferentiated fat cells ameliorates Habu Snake Venom-induced chronic renal dysfunction in tenascin-C-deficient mice. Nephron Experimental Nephrology 110(3):e91-98, 2008
  5. Oki Y, Watanabe S, Endo T, Kano K. Mature adipocyte-derived dedifferentiated fat cells can trans-differentiate into osteoblasts in vitro and in vivo only by all-trans retinoic acid. Cell Structure and Function 33(2):211-222, 2008
  6. Kazama T, Fujie M, Endo T, Kano K. Mature adipocyte-derived dedifferentiated fat cells can transdifferentiate into skeletal myocytes in vitro. Biochemical and Biophysical Research Communications 377(3):780-785, 2008
  7. Nobusue H, Endo T, Kano K. Establishment of a preadipocyte cell line derived from mature adipocytes of GFP transgenic mice and formation of adipose tissue. Cell and Tissue Research 332(3):435-446, 2008
  8. Sakuma T, Matsumoto T, Kano K, Fukuda N, Obinata D, Yamaguchi K, Yoshida T, Takahashi S, Mugishima H. Mature adipocyte derived dedifferentiated fat cells can differentiate into smooth muscle-like cells and contribute to bladder tissue regeneration. Journal of Urology 182 (1):355-365, 2009.
  9. Matsubara Y, Kano K, Kondo D, Mugishima H, Matsumoto T. Differences in adipocytokines and fatty acid composition between two adipocyte fractions of smaall and large cells in high-fat diet-induced obese mice. Annals of Nutrition & Metabolism 54(4):258-267, 2009
  10. Matsumura M, Fukuda N, Kobayashi N, Umezawa H, Takasaka A, Matsumoto T, Yao EH, Ueno T, Negishi N. Effects of atrovatatin on angiogenesis in hidlimb ischemia and endothelial progenitor cell formation in rats. Journal of Atherosclerosis and Thrombosis 16(4):319-326, 2009
  11. Jumabay M, Matsumoto T, Yokoyama SI, Kano K, Masuko T, Mitsumata M, Saito S, Hirayama A, Mugishima H, Fukuda N. Dedifferentiated fat cells convert to cardiomyocytes phenotype and repair infarcted cardiac tissue in rats. Journal of Molecular and Cellular Cardiology 47(5):565-575, 2009
  12. Obinata D, Matsumoto T, Ikado Y, Sakuma T, Kano K, Fukuda N, Yamaguchi K, Mugishima H, Takahashi S. Transplantation of mature adipocyte-derived dedifferentiated fat (DFAT) cells improves urethral sphincter contractility in a rat model. International Journal of Urology 18(12):827-834, 2011
  13. Ono H, Oki Y, Bono H, Kano K. Gene expression profiling in multipotent DFAT cells derived from mature adipocytes. Biochemical and Biophysical Research Communications 407(3):562-567, 2011
  14. 松村昌治, 松本太郎, 加野浩一郎, 麦島秀雄:家兎下肢虚血モデルに対する脱分化脂肪細胞(DFAT)自家移植の効果. 日大医学雑誌 72(2) 86-92, 2013
  15. Kikuta S, Tanaka N, Kazama T, Kazama M, Kano K, Ryu J, Tokuhashi Y, Matsumoto T. Osteogenic effects of dedifferentiated fat (DFAT) cell transplantation in rabbit models of bone defect and ovariectomy (OVX)-induced osteoporosis. Tissue Engineering Part A 19(15-16):1792-1802, 2013
  16. Kono S, Kazama T, Kano K, Harada K, Uechi M, Matsumoto T. Phenotypic and functional properties of feline dedifferentiated fat cells and adipose-derived stem cells. The Veterinary Journal 199(1):88-96, 2014
  17. Matsumine H, Takeuchi Y, Sakaki R, Kazama T, Kano K, Matsumoto T, Sakurai H, Miyata M, Yamato M. Adipocyte-derived and dedifferentiated fat cells promoting facial nerve regeneration in a rat model. Plastic and Reconstructive Surgery 134(4): 686-697, 2014
  18. Mikami Y, Matsumoto T, Kano K, Toriumi T, Somei M, Honda MJ, Komiyama K. Current status of drug therapies for osteoporosis and the search for stem cells adapted for bone regenerative medicine. Anatomical Science International 89(1):1-10, 2014
  19. Yamada H, Ito D, Oki Y, Kitagawa M, Matsumoto T, Watari T, Kano K. Transplantation of mature adipocyte-derived dedifferentiated fat cells promotes locomotor functional recovery by remyelination and glial scar reduction after spinal cord injury in mice. Biochemical and Biophysical Research Communications 454(2):341-346, 2014
  20. Nobusue H, Onishi N, Shimizu T, Sugihara E, Oki Y, Sumikawa Y, CHiyoda T, Akashi K, Saya H, Kano K. Regulation of MLK1 via actin cytoskeleton dynamics drives adipocyte differentiation. Nature Communications 5:3368, 2014
  21. Soejima K, Kashimura T, Asami T, Kazama T, Matsumoto T, Nakazawa H. Effects of mature adipocyte-derived dedifferentiated fat (DFAT) cells on generation and vascularization of dermis like tissue after artificial dermis grafting. Journal of Plastic Surgery and Hand Surgery 49(1): 25-31, 2015
  22. Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S, Kazama T, Matsumoto T, Takeuchi S. Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology. PLoS One 10(3):e0119010, 2015
  23. Asami T, Soejima K, Kashimura T, Kazama T, Matsumoto T, Morioka K, Nakazawa H. Effects of combination therapy using basic fibroblast growth factor and mature adipocyte-derived dedifferentiated fat (DFAT) cells on skin graft revascularization. Journal of Plastic Surgery and Hand Surgery 49(4):229-233, 2015
  24. Maruyama T, Fukuda N, Matsumoto T, Kano K, Endo M, Kazama M, Kazama T, Ikeda J, Matsuda H, Ueno T, Abe M, Okada K, Soma M, Matsumoto K, Kawachi H. Systematic implantation of dedifferentiated fat cells ameliorated monoclonal antibody 1-22-3-induced glomerulonephritis by immunosuppression with increasing in TNF-stimulated gene 6. Stem Cell Research & Therapy 6(1): 80, 2015
  25. 渡邉拓史, 松本太郎, 萩倉一博, 風間智彦, 高橋昌里. 脱分化脂肪細胞(DFAT)における血管新生効果の検討. 日大医学雑誌 74(5):238-245, 2015
  26. 大瀧宗典, 松本太郎, 加野浩一郎, 徳橋泰明. ヒト脱分化脂肪細胞の軟骨分化能の検討. 日大医学雑誌 74(5):246-252, 2015
  27. Kashimura T, Soejima K, Asamai T, Kazama T, Matsumoto T, Nakazawa H. The effect of mature adipocyte-derived dedifferentiated fat (DFAT) cells on a dorsal skin flap model. Journal of Investigative Surgery 29(1):6-12, 2016
  28. Nakamura T, Kazama T, Nagaoka Y, Inamo Y, Mugishima H, Takahashi S, Matsumoto T. Influence of donor age and passage number on angiogenic activity in human adipose-derived stem cell-conditioned media. Journal of Stem Cell Research &Therapy 5:307, 2015
  29. Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, kazama T, Oki Y, Tamura Y, Tonogi M, Isokawa K, Shimizu N, Honda M. Small buccal fat pad cells have high osteogenic differentiation potential. Tissue Engineering Part C 22(3): 250-259, 2016
  30. Taniguchi H, Kazama T, Hagikura K, Yamamoto C, Kazama M, Nagaoka Y, Matsumoto T. An efficient method to obtain dedifferentiated fat cells. Journal of Visualized Experiments Jul 15;(113), e54177, 2016.
  31. Akita D, Kano K, Saito-Tamura Y, Mashimo T, Sato-Shionome M, Tsurumachi N, Yamanaka K, Kaneko T, Toriumi T, Arai Y, Tsukimura N, Matsumoto T, Ishigami T, Isokawa K, Honda M. Use of rat mature adipocyte-derived dedifferentiated fat cells as a cell source for periodontal tissue regeneration. Frontiers in Physiology 7: 50, 2016
  32. Ikado Y, Obinata D, Matsumoto T, Murata Y, Kano K, Fukuda N, Yamaguchi K, Takahashi S. Transplantation of mature adipocyte-derived dedifferentiated fat cells for treatment of vesicoureteral reflux in a rat model. International Urology and Nephrology 48(12): 1951-1960, 2016.
  33. 副島一孝, 樫村勉, 地家豊治, 風間智彦, 松本太郎, 仲沢弘明. 皮膚再建の再生医療. 日大医学雑誌 75(2):74-80, 2016
  34. Murai T, Matsumoto T, Ishige M, Kazama T, Kano K, Mugishima H. Effect of dedifferentiated fat cell transplantation in a mouse model of acute graft-versus-host disease. J Nihon Univ Med 76(4-5): 187-194, 2017
  35. Toyama K, Kano K, Matsumoto T, Hirayama A. Study of cardiac muscle differentiation potential in epicardial adipose-derived dedifferentiated fat cells (DFAT). J Nihon Univ Med 76(4-5): 175-185, 2017
  36. Suzuki D, Akita D, Tsurumachi N, Kano K, Yamanaka K, Kaneko T, Kawano E, Iguchi S, Toriumi T, Arai Y, Matsumoto T, Isokawa K, Sato S, Honda M. Transplantation of mature adipocyte-derived dedifferentiated fat cells into three-wall defects in the rat periodontium induces tissue regeneration. Journal of Oral Science 59(4):611-620, 2017
  37. Mikrogeorgiou A, Kondo T, Hattori T, Sugiyama Y, Nakanishi K, Tsuji M, Kazama T, Kano K, Matsumoto T, Hayakawa M, Sato Y. Dedifferentiated fat cells, as a novel source for cell therapy to target neonatal hypoxic ischemic encephalopathy. Developmental Neuroscience 39(1-4):273-286, 2017
  38. Nakayama E, Matsumoto T, Kazama T, Kano K, Tokuhashi Y. Transplantation of dedifferentiated fat cells promotes intervertebral disc regeneration in a rat intervertebral disc degeneration model. Biochemical and Biophysical Research Communications 493(2):1004-1009, 2017.
  39. Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama T, Oki Y, Saito-Tarura Y, Tonogi M, Shimizu N, Honda M. Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad. Journal of Oral Science 60(1):14-23, 2018
  40. Shimizu M, Matsumoto T, Kikuta S, Ohtaki M, Kano K, Taniguchi H, Saito S, Nagaoka M, Tokuhashi Y. Transplantation of dedifferentiated fat cell-derived micromass pellets contributed to cartilage repair in the rat osteochondral defect model. Journal of Orthopaedic Science 23(4):688-696, 2018
  41. Soejima K, Kashimura T, Kazama T, Matsumoto T, Nakazawa H. Effect of mature adipocyte-derived fat (DFAT) cells on formation of basement membrane after cultured epithelial autograft on artificial dermis. Plastic and Reconstructive Surgery 143(5):983e-992e, 2019
  42. Sakuma T, Matsumoto T, Kazama T, Tokuhashi Y. Analysis of cell-to-cell interactions between intervertebral nucleus pulposus cells and dedifferentiated fat cells using a coculture system. J Nihon Univ Med 78(4-5): 285-293, 2019
  43. Fujimaki H, Matsumine H, Osaki H, Ueta Y, Kamei W, Shimizu M, Hashiomto K, Fujii K, Kazama T, Matsumoto T, Niimi Y, Miyaka M, Sakurai H. Dedifferentiated fat cells in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration. Regenerative Therapy 11:240-248, 2019
  44. Yamazaki T, Kishimoto T, Leszcznski P, Sadakane K, Kenmotsu T, Watanabe H, Kazama T, Matsumoto T, Yoshikawa K, Taniguchi H. Construction of 3D cellular composites with stem cells derived from adipose tissue and endothelial cells by use of optical tweezers in a natural polymer solution. Materials. 2019, 12(11) pii: E1759, doi:10.3390/ma12111759
  45. Nakano R, Kitanaka T, Namba S, Kitanaka N, Sato M, Shibukawa Y, Masuhiro Y, Kano K, Matsumoto T, Sugiya H. All-trans retinoic acid induces reprogrammig of canine dedifferentiated cells into neuron-like cells. PLoS One. 2020, 15(3):e0229892, doi:10.1371/journal.pone.0229892.
  46. Watanabe H, Goto S, Kato R, Komiyama S, Nagaoka Y, Kazama T, Yamamoto C, Li Y, Konuma N, Hagikura K, Matsumoto T. The neovascularization effect of dedifferentiated fat cells. Scientific Reports 10(1):9211, 2020
  47. Ishioka S, Hosokawa T, Ikeda T, Konuma N, Kaneda H, Ohashi K, Furuya T, Masuko T, Taniguchi H, Kano K, Koshinaga T, Matsumoto T. Therapeutic potential of mature adipocyte-derived dedifferentiated fat cells for inflammatory bowel disease. Pediatric Surgery International 36(7):799-807, 2020
  48. Kawachi H, Matsumoto T, Kazama T, Yamamoto C, Kano K, Maeda H, Kono K, Shiono M. Tanaka M. The effect of autologous dedifferentiated fat cell transplantation in a pig model of hindlimb ischemia. Journal of Nihon University School of Medicine 79(3): 133-143, 2020
  49. Yamada T, Matsumoto T, Kazama T, Arai Y, Kano K, Tokuhashi Y, Nakanishi K. Promoting effect of dedifferentiated fat cell transplantation on bone formation in a mouse femoral fracture model. Journal of Nihon University School of Medicine 79(5):265-273, 2020
  50. Hashimoto M, Uekusa S, Ono K, Goto S, Hosokawa T, Koshinaga T, Matsumoto T. Establishment of induced pluripotent stem cells from dedifferentiated adipocytes. Journal of Nihon University School of Medicine 79(5):275-286, 2020
  51. Tanimoto K, Matsumoto T, Nagaoka Y, Kazama T, Yamamoto C, Kano K, Nagaoka M, Saito S, Tokuhashi Y, Nakanishi K. Phenotypic and functional properties of dedifferentiated fat cells derived from infrapatellar fat pad. Regenerative Therapy 19:35-46, 2022
  52. Akita D, Kazama T, Tsukimura N, Taniguchi Y, Takahashi R, Arai Y, Tsurumachi-Iwasaki N, Yasuda H, Okubo T, Kano K, Matsumoto T, Honda M. Transplantation of mature adipocyte-derived dedifferentiated fat cells facilitates periodontal tissue regeneration of class II furcation defects in miniature pigs. Materials 15(4):1311, 2022
  53. Murata Y, Obinata D, Matsumoto T, Ikado Y, Kano K, Fukuda N, Yamaguchi K, Takahashi S. Urethral injection of dedifferentiated fat cells ameliorates sphincter damage and voiding dysfunction in a rat model of persistence stress urinary incontinence. International Urology and Nephrology 2022 In press 54(4):789-797, 2022
  54. Utsunomiya K, Maruyama T, Shimizu S, Matsumoto T, Endo M, Kobayashi H, Kano K, Abe M, Fukuda N. Implantation of dedifferentiated fat cells ameliorated antineutrophil cytoplasmic antibody glomerulonephritis by immunosuppression and increases in tumor necrosis factor-stimulated gene-6. Stem Cell Research & Therapy 13(1): 319, 2022
  55. Hidaka A, Uekusa S, Hosokawa T, Kaneda H, Kazama T, Hagikura K, Uehara S, Koshinaga T, Matsumoto T. Effects of dedifferentiated fat cells on neurogenic differentiation and cell proliferation in neuroblastoma cells. Pediatric Surgery International 39(1): 58, 2022
  56. 小山公行, 松本太郎, 宮方啓行, 風間智彦, 上井浩, 徳橋泰明, 中西一義: 椎間板針穿刺による椎間板変性ラットに対する脱分化脂肪細胞静脈内投与の治療効果. 日大医学雑誌 81(5):273-281, 2022
  57. Sawada H, Kazama T, Nagaoka Y, Arai Y, Kano K, Uei H, Tokuhashi Y, Nakanishi K, Matsumoto T. Bone marrow-derived dedifferentiated fat cells exhibit similar phenotype as bone marrow mesenchymal stem cells with high osteogenic differentiation and bone regeneration ability. Journal of Orthopaedic Surgery and Research 18(1):191, 2023
  58. Mimatsu H, Onoda A, Kazama T, Nishijima K, Shinoyama Y, Go S, Ueda K, Takahashi Y, Matsumoto T, Hayakawa M, Sato Y. Dedifferentiated fat cells administration ameliorates abnormal expression of fatty acids metabolism-related protein expressions and intestinal tissue damage in experimental necrotizing enterocolitis. Scientific Reports 13(1):8266, 2023

総説・著書

  1. 加野浩一郎, 松本太郎, 遠藤克, 麦島秀雄: 成熟脂肪細胞由来の前駆脂肪細胞株DFATを用いた新規の再生医療用ドナー細胞の開発. 日本再生医療学会雑誌, 5:112-118, メディカルレビュー社, 2006
  2. 山元智衣, 松本太郎, 加野浩一郎, 福田昇, 松本紘一, 麦島秀雄：脂肪細胞から尿細管上皮へのtransdifferentiation. 腎臓, 28:185-189, 財団法人　日本腎臓財団, 2006
  3. 松本太郎：再生医療実現化に向けた取り組み. 日大医学雑誌 65 (3) 244-246, 2006
  4. 松本太郎, 麦島秀雄: 心血管再生に向けた基礎研究. 高血圧（上巻）, 64別冊5:547-551, 日本臨床社, 大阪, 2006.
  5. 松本太郎, 福田昇: 脱分化脂肪細胞(DFAT)を細胞源とする再生医療, 医学のあゆみ, 230(8):553-555, 医歯薬出版株式会社, 2009. 8
  6. 清水学, 松本太郎: 幹細胞(stem cell) 〜現在と今後の展望〜脊髄脊椎ジャーナル 22:324-325, 三輪書店, 2009
  7. 大日方大亮, 松本太郎, 高橋悟: 脱分化脂肪細胞(DFAT)による尿道平滑筋再生, 排尿障害プラクティス, 18(4):7-14, メディカルレビュー社, 2010. 12
  8. 松本太郎: 脱分化脂肪細胞 (DFAT)（脂肪組織由来幹細胞療法コンセンサス会議，脂肪組織由来幹細胞療法の基礎）, 臨床評価, 38(4):761-765, 臨床評価刊行会, 2011. 5
  9. 松本太郎：脱分化脂肪細胞を細胞源とする再生医療. 呼吸, 30(9):782-787, Respiration Research Foundation, 2011. 9
  10. 松本太郎: 脱分化脂肪細胞(DFAT)の特性と細胞治療への応用. 医学のあゆみ（脂肪由来幹細胞の臨床応用への展開）, 242(4):326-331, 医歯薬出版株式会社, 東京, 2012. 7
  11. 松本太郎：話題 新規治療用細胞としての脱分化脂肪細胞. 日大医学雑誌 72(3) 186, 2013
  12. 松本太郎, 風間智彦: 再生医療で用いられる細胞種の培養<4> 脂肪細胞. 再生医療における臨床研究と製品開発, p181-187, 技術情報協会, 東京, 2013. 9
  13. 松本太郎：再生医療：基礎と臨床. 日大医学雑誌75(2) 55, 2016
  14. 村田保貴, 井門祐一郎, 大日方大亮, 咲間隆裕, 山口健哉, 松本太郎, 高橋悟: 脱分化脂肪細胞を用いた下部尿路の再生医療について. 泌尿器外科　(特集　下部尿路機能再生医療の現況), 29(1): p 9-13, 医学図書出版株式会社, 埼玉, 2016. 1
  15. 丸山高史, 松本太郎: 腎疾患に対する脱分化脂肪細胞を用いた細胞治療. 臨床免疫・アレルギー科, 65(6):593-598, 科学評論社, 東京, 2016. 6
  16. 小沼憲祥, 松本太郎: 脱分化脂肪細胞(DFAT)を細胞源とする再生医療. 小児外科（特集　小児外科領域の先端的医療の展開(I):再生医療の最前線）, 49(5): 445-448, 東京医学社, 東京, 2017. 5